en خون شناسی آزمایشگاهی و بانک خون Laboratory hematology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Polycythemia Vera (PV) is a myeloproliferative neoplasm (MPN) characterized by the overactivity of erythroid progenitors, leading to excessive red blood cell (RBC) production. More than 90% of PV cases harbor a Janus kinase 2 (JAK2) gene mutation. This study aimed to assess the prevalence of JAK2 mutations in individuals with elevated hemoglobin (Hb) levels referred to the laboratory by physicians.<br> <b>Methods:</b> In this descriptive cross-sectional study, genomic DNA from 72 patients was analyzed for JAK2 mutations using a TaqMan-specific probe.<br> <b>Results:</b> Of the 72 patients, 24 (33.3%) were women and 48 (66.6%) were men. JAK2 mutations were detected in 33 cases (45.5%), while 39 (54.2%) were negative. Notably, 15 of 24 female patients (62.5%) tested positive for the JAK2 mutation, compared to 18 of 48 male patients (37.5%).<br> <b>Conclusion</b>: Our findings suggest that screening for JAK2 mutations is particularly important in women with above-normal Hb levels.</span></span></div> Polycythemia vera, JAK2 protein, human, Mutation, Hemoglobins, Women 30 33 http://mlj.goums.ac.ir/browse.php?a_code=A-10-644-4&slc_lang=en&sid=1 Zeinab Siahmargoie siahmargoie@yahoo.com 100319475328460030519 0009-0008-8318-5878 No Iranian Blood Transfusion Organization, Gorgan Branch, Iran Mohammad Taher Hojjati mthhematology@gmail.com 100319475328460030520 100319475328460030520 Yes Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Hadi Bazzazi hadi.bazzazi@gmail.com 100319475328460030521 100319475328460030521 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Khodaberdi Kalavi kalavi@goums.ac.ir 100319475328460030522 100319475328460030522 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Mana Zakeri Mana_zakeri1165@yahoo.com 100319475328460030523 100319475328460030523 No Department of Medical Genetics, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Hadi Joshaghani 100319475328460030524 0009-0001-7764-4125 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran