en ایمنی شناسی Immunology تحقيقي Original Paper <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Sepsis stands as a leading cause of mortality among critically ill patients in intensive care units (ICUs). Bacterial infections, including sepsis, upregulate Neutrophilic cluster of differentiation 64 (nCD64) expression on activated polymorphonuclear leukocytes (PMNs). Prompt diagnosis of sepsis is crucial for initiating timely and targeted treatment. Consequently, a rapid laboratory test with high specificity for sepsis in adults would significantly aid therapeutic decision-making and help reduce the overuse of antibiotics.<br> <b>Methods:</b> This study enrolled 40 sepsis patients diagnosed according to the Sepsis-3 definition. For biomarker evaluation, 2 mL blood samples were collected from each patient in both ethylenediaminetetraacetic acid (EDTA) and plain vials. In addition, nCD64 was analyzed using flow cytometry, high-sensitivity C-reactive protein (hs-CRP) via nephelometry, and procalcitonin (PCT) using chemiluminescence.<br> <b>Results:</b> For sepsis prediction, nCD64 demonstrated a positive predictive value (PPV) of 92.68% and a negative predictive value (NPV) of 94.87%. A receiver operating characteristic (ROC) curve was generated to assess the diagnostic accuracy of nCD64 (&ge; 1.8), hS-CRP (&ge; 3 mg/L), and PCT (&ge; 0.4 ng/mL). The area under the curve (AUC) for nCD64 was highest at 0.938 (95% confidence interval [CI] = 0.876-0.999), followed by hS-CRP at 0.888 (95% CI = 0.807-0.968) and PCT at 0.850 (95% CI = 0.759-0.941).<br> <b>Conclusion</b>: These findings strongly suggest that nCD64 determination is a valuable diagnostic tool for identifying infections in patients with septic syndrome. Its performance appears to be superior to that of hs-CRP and PCT.</span></span></div> Procalcitonin, C-Reactive Protein, Sepsis, Biomarkers 17 21 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1608-1&slc_lang=en&sid=1 Anuradha Saini anuradhasaini582@gmail.com 100319475328460030590 00009-0009-3245-9098 No Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India Anjali Sharma anjalisharmapath@gmail.com 100319475328460030591 100319475328460030591 Yes Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India Mukul Singh drmukulsingh@gmail.com 100319475328460030592 100319475328460030592 No Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India Shaily Goyal shailygl19@gmail.com 100319475328460030593 100319475328460030593 No Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India Maninder Narang mnarang.med@gmail.com 100319475328460030594 0009-0009-0873-0669 No Department of Medicine, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India Sunil Ranga sunilran18@yahoo.co.in 100319475328460030595 100319475328460030595 No Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India