en بیوشیمی Biochemistry Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> DNA glycation damages DNA by inducing strand breaks, mutations, and ultimately changes in gene expression, which is considered a main factor in the pathogenesis of diabetes and its complications. Therefore, antiglycation agents have become the focus of recent research for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of <i>Artemisia sieberi</i> (<i>A. sieberi</i>) leaf extract, this study aimed to determine the effect of the ethanolic extract of <i>A. sieberi</i> on glucose-mediated DNA glycation for the first time.<b><span style="font-size:9.0pt"></span></b><br> <b>Methods:</b> DNA was incubated with glucose in the presence or absence of <i>A. sieberi</i> for 4 weeks. The inhibitory or facilitatory effects of <i>A. sieberi</i> on DNA structural changes were studied by various techniques. These techniques included UV&ndash;Vis, fluorescence spectroscopy, circular dichroism (CD), and agarose gel electrophoresis.<br> <b>Results:</b> The findings of UV&ndash;Vis and fluorescence spectroscopy showed that <i>A. sieberi</i> decreased DNA-AGE (Advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA were decreased in the presence of <i>A. sieberi</i>.<b><span style="font-size:9.0pt"></span></b><br> <b>Conclusion</b>: Thus, <i>A. sieberi</i> has beneficial effects against DNA glycation and could be a promising agent for ameliorating the adverse effects of glycation in the presence of glucose and in conditions of raised blood glucose, such as diabetes, after confirmation in further studies.<span style="font-size:9.0pt"></span></span></span><br> &nbsp;</div> Glycation, Artemisia Sieberi, DNA, Glucose, Advanced glycation end products 34 37 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1798-2&slc_lang=en&sid=1 Parisa Hasanein parisa.hasanein@gmail.com 100319475328460031638 100319475328460031638 Yes Department of Biology, Faculty of Science, Bu Ali Sina University, Hamedan, Iran Mehrnush Sotudeh mahsamaleki89@gmail.com 100319475328460031639 100319475328460031639 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran Mousa Bohlooli mousa.bohlooli@gmail.com 100319475328460031640 100319475328460031640 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran; Department of Cell and Molecular Sciences, Kharazmi University, Tehran, Iran Mohammad Haddadi mhadadi@gmail.com 100319475328460031641 100319475328460031641 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran