fa تحقيقي Original Paper <div> <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective:</strong> Antibiotic-resistant <em>Staphylococcus aureus</em> strains have become a problem in treatment of infections caused by <em>S. aureus</em>. This study aimed to evaluate antibiotic resistance in <em>S. aureus</em> isolates from raw milk and detect <em>femA </em>gene in these isolates, as a confirmatory test for identification of <em>S. aureus</em> species.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> This cross-sectional study was performed on 110 raw milk samples. After culture in Cooked Meat broth, presence of <em>S. aureus</em> in grown colonies was confirmed in accordance with Iranian National Standard, No. 1194. Antibiotic resistance was then evaluated according to guidelines recommenced by the Clinical Laboratory Standards Institute. <em>FemA-specific</em> polymerase chain reaction was performed on antibiotic-resistant strains using specific primers and standard strains to differentiate <em>S. aureus</em> from other species.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> <em>S. aureus</em> were found in 43 (39.09%) of the 110 collected samples. Among these isolates, 79.07% and 76.75% were phenotypically resistant to penicillin and ceftazidime, respectively. In addition, the <em>femA</em> gene was detected in all isolates.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion:</strong> The results of this study show a high prevalence of resistance to penicillin and ceftazidime among <em>S. aureus</em> strains isolated from raw milk.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: </strong><em>Staphylococcus aureus</em>, Antibiotic Resistance, PCR.</p> </div> Staphylococcus aureus, Antibiotic Resistance, PCR. 40 45 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-413&slc_lang=fa&sid=1 Pouya Khodadadi hosseinansari62@gmail.com 100319475328460011183 100319475328460011183 Yes Department of Microbiology, Islamic Azad University, Masjed-Soliman Branch, Masjed-Soliman, Iran Mehdi Bizhanzadeh 100319475328460011184 100319475328460011184 No Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Akram Najafi 100319475328460011185 100319475328460011185 No Department of Marine Microbiology, Persian Gulf Marine Biotechnology Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran Vajiheh Zarinpour 100319475328460011186 100319475328460011186 No Department of Genetic, Faculty of Science, Islamic Azad University, Damghan Branch, Damghan, Iran Abdolali Moshfe 100319475328460011187 100319475328460011187 No Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Hossein Ansari 100319475328460011188 100319475328460011188 No Department of Microbiology, Islamic Azad University, Masjed-Soliman Branch, Masjed-Soliman, Iran