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Showing 3 results for Glycated Hemoglobin
Comparison of glycated albumin and glycated Hemoglobin in type 2 diabetic patients with end-stage of renal disease
Israa Elgaily, Abdelkarim A. Abdrabo,
Volume 18, Issue 5 (9-2024)
Abstract
Background: Various analytes are used to assess glycemic control in laboratory medicine. Glucose measurements show current glucose levels, but sample stability can be influenced by diet and stress. Hemoglobin A1c (HbA1c) is the best marker for long-term control but can be affected by elevated urea levels. This study compared glycated albumin (GA) and HbA1c in diabetic patients undergoing hemodialysis.
Methods: A comparative cross-sectional study was conducted with a sample size of 280 volunteers. Among these, there were 115 diabetic patients with end-stage renal disease (ESRD), 95 diabetic patients without ESRD, and 75 non-diabetic patients with ESRD. Laboratory measurements included HbA1c, GA, urea, and creatinine, assessed using standard laboratory techniques. Data analysis was carried out using SPSS statistical software.
Results: Levels of HbA1c were lower in diabetic patients with ESRD compared to diabetic patients without ESRD. In contrast, GA levels were higher in diabetics with ESRD. A significant negative association was observed between HbA1c levels and urea levels. However, creatinine levels were not associated with either HbA1c or GA.
Conclusion: The estimation of glycated hemoglobin levels can be affected by high blood urea. Therefore, GA may be a better glycemic indicator for diabetic patients with ESRD.

Relationships between serum lipid profile and HbA1c levels in patients with diabetes
Habibeh Sadat Mosavi , Mohammad Taher Hojjati , Khodaberdi Kalavi,
Volume 18, Issue 5 (9-2024)
Abstract
Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia, disturbed lipid metabolism, and cardiovascular disease (CVD). Poor glycemic control results in lipid and lipoprotein abnormalities, suggesting that dyslipidemia is secondary to insulin resistance or factors closely related to insulin resistance. Glaciated hemoglobin (HbA1c) is a routinely used marker for long-term glycemic control. This form of hemoglobin is chemically processed through post-translational glycosylation that reflects the plasma glucose concentration during the last two to three months.
Methods: This cross-sectional study was conducted on blood samples from 592 cases referred to Dezyani Specialty Clinic, affiliated with the Golestan University of Medical Sciences. In patients with diabetes mellitus (DM) and non-diabetic individuals, hemoglobin A1c (HbA1c), total cholesterol (T-chol), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were measured.
Results: In the study, there were 460 female participants (76.9%) and 132 male participants (23.1%). Among them, 403 (67.4%) were diabetic patients, and 193 (32.4%) were non-diabetic. HbA1c levels were significantly lower in younger individuals than in middle-aged participants (p=0.000). No significant difference was found between middle-aged and older individuals (p>0.121). LDL levels were significantly lower in the young group than in the middle-aged group (p<0.001). Total cholesterol (TC) was also significantly lower in the young group compared to middle-aged and older groups (p<0.001).
Conclusion: HbA1c can be a helpful lipid and glycemic control marker in diabetes.
 

Comparative evaluation of HPLC and nephelometry for HbA1c measurement: Diagnostic agreement and clinical utility
Lohitha Polisetty , Battula Roopa Neeharika ,
Volume 20, Issue 2 (6-2026)
Abstract
Background: Glycated haemoglobin (HbA1c) reflects estimated average blood glucose levels over a period of 2-3 months and is essential for both diagnosing and managing diabetes. High-performance liquid chromatography (HPLC), particularly ion-exchange HPLC, is widely regarded as the reference and gold standard method for HbA1c assessment, whereas nephelometry is commonly adopted in clinical laboratories because it provides faster results. This study compared the accuracy, reliability, and clinical applicability of these two methods.
Methods: A total of 50 patients diagnosed with diabetes mellitus and attending a tertiary care hospital were included in this cross-sectional study. For each participant, HbA1c levels were estimated using both ion-exchange HPLC and nephelometric techniques. Data analysis involved descriptive statistics, Pearson’s correlation, the intraclass correlation coefficient (ICC), Bland-Altman plots, and receiver operating characteristic (ROC) curve evaluation. A paired t-test was performed to determine statistical significance, and values with p < 0.05 were considered statistically significant.
Results: The mean HbA1c values obtained by HPLC (6.2% ± 1.5) and nephelometry (6.3% ± 1.4) were closely aligned. A strong correlation was observed between the two techniques (r = 0.96, p < 0.01), and the intraclass correlation coefficient also indicated excellent concordance (ICC = 0.96). Bland-Altman plotting revealed only a slight bias, with a mean difference of 0.09%. ROC curve evaluation showed that both methods exhibited good diagnostic capability, although HPLC achieved marginally higher sensitivity (90%) and specificity (92%) than nephelometry (88% and 91%, respectively). While nephelometry offered faster processing, its diagnostic accuracy was slightly lower.
Conclusion: Although HPLC demonstrated superior diagnostic accuracy and method agreement, nephelometry offers operational advantages in high-volume settings. Despite the high correlation between the two methods, their interchangeability should be approached with caution, particularly in borderline cases. Further studies with larger and more diverse cohorts, as well as evaluation of potential confounding factors such as hemoglobin variants, are warranted.

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