en بیوشیمی Biochemistry Research Article Research Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><b>Background:</b> Aflatoxin B1 (AFB1) is one of the most important mycotoxins that contaminate food worldwide. Long-term consumption of foods contaminated with AFB1 endangers human health. Detoxification of AFB1 from food improves community health. A Specific approach to aflatoxin reduction is the use of probiotics. Kefir drink is a strong probiotic. The purpose of this study was to investigate the protective effect of kefir drink on AFB1-induced hepatic injury in adult male rats<br> <b>Methods:</b> In this experimental study, 24 adult rats weighing between 150 and 200 g were used. The rats were randomly divided into 4 groups: 1) control, 2) AFB1 (50 &mu;g/kg body weight), 3) kefir drink (10 mL/kg body weight), and 4) AFB1 + kefir drink. Aflatoxin and kefir drink received through oral gavage. At the end of the experiment (8 weeks), blood and liver samples were collected for different assays. Liver function tests and histopathological examinations were performed. Data were analyzed using 1-way analysis of variance (ANOVA) and at a significance level of <0.05.<br> <b>Results:</b> Aflatoxin B1 significantly increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (T.Bili), as well as decreased total protein (T.P) content, compared to the control group (P < 0.05). Aflatoxin B1 induced histological changes in the liver. The results obtained from the groups treated with kefir drink with and without AFB1 were not significantly different from the control group. Histopathological changes were not found in groups treated with kefir drink with and without AFB1.<br> <b>Conclusion</b>: The consumption of kefir drink reduced AFB1-induced disruptions in rats&rsquo; livers.</span></span> Kefir , Aflatoxin B1, Probiotics, liver functions, histopathological changes. 17 19 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1320-1&slc_lang=en&sid=1 Seyed ahmad Sajjadi 100319475328460031914 100319475328460031914 No Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Zahra Moosavi 100319475328460031915 0000.0002.8388.2699 No Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Farhad Niknejad 100319475328460031916 100319475328460031916 No Department of Medical Parasitology and Mycology, Golestan University of Medical Sciences, Gorgan, Iran Abdollah Jamshidi ajamshid@ferdowsi.um.ac.ir 100319475328460031917 100319475328460031917 Yes Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran