fa شناسایی گونه های مالاسزیایی جدا شده از بیماران مبتلا به درماتیت سبورئیک به روش PCR-RFLP در شهر اراک تحقيقي Original Paper Abstract Background and Objective: Malassezia that is a part of normal flora is lipophilic yeast involved in a variety of skin diseases such as seborrheic dermatitis, pityriasis versicolor, atopic dermatitis and psoriasis. Seborrheic dermatitis affects most often the sebaceous-gland-rich areas of skin such as face, scalp, and parts of the upper trunk. Dandruff is a mild variant of seborrheic dermatitis characterized by scaling. In this study, Malassezia species causing dandruff were identified. Material and Methods: In this descriptive study, the samples (n= 60) from participants with dandruff were examined under a microscope using 10% KOH solution and cultured in Leeming and Notman ager medium. DNA Extraction was performed from colonies by glass bead and the Malassezia genus, and species were detected by CfoI enzyme using PCR-RFLP method Results: Of 60, 40 (66.6%) were positive for Malassezia yeast. The positive samples in direct examination grew in culture medium. Malassezia species isolated were Malassezia globosa (25 cases), Malassezia restricta (10 cases), Malassezia furfur (3 cases) and Malassezia sympodialis (2 cases). Conclusions: In most studies, the Malassezia species were identified as the agents causing seborrheic dermatitis. In our study, Malassezia globosa was isolated as a dominant species. Keywords: Seborrheic Dermatitis, Malassezia SPP, Arak Seborrheic Dermatitis, Malassezia SPP, Arak 84 89 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-274&slc_lang=fa&sid=1 Mohajerani, MR حمید رضا مهاجرانی 100319475328460012697 100319475328460012697 No Islamic Azad University, Arak Branch, Iran دانشگاه علوم تحقیقات اراک Sarikhani, A احمد ساریخانی 100319475328460012698 100319475328460012698 No Islamic Azad University, Arak Branch, Iran دانشگاه آزاد اراک Gandomani, M مولود گندمانی 100319475328460012699 100319475328460012699 No Islamic Azad University, Arak Branch, Iran دانشگاه علوم پزشکی اراک Eslamirad, Z زهرا اسلامی راد 100319475328460012700 100319475328460012700 No Arak University of Medical Sciences, Arak, Iran دانشگاه علوم پزشکی اراک Mosayebi, M مهدی مسیبی 100319475328460012701 100319475328460012701 No Arak University of Medical Sciences, Arak, Iran دانشگاه علوم پزشکی اراک Didehdar, M مجتبی دیده دار didehdar_m@Arakmu.ac.ir 100319475328460012702 100319475328460012702 Yes Arak University of Medical Science, Arak, Iran دانشگاه علوم پزشکی اراک